MT, CLS, and other lab people: How exactly does a detection of HIV viral load test work? ?
I know that the most common methods for this test are the Quantiplex and Amplicor PCR or DNA Versant branco (bDNA) test. I know that a viral load test measures the amount of HIV in the One milliliter of blood, but someone can explain the inspection procedures? Oh, and do not be afraid to use the jargon of the lab I am a clinical laboratory science / student Medical Technology. I now work as part of a research project for one of my course. Thank you.
Viral load tests are performed using PCR. The chain reaction amplified DNA polymerase (or in the case of HIV RNA). In this way, you can take small amounts of viral RNA is present in the blood and quantify. The same test also known as the test NAAT (nucleic acid). To learn more about the PCR test, read the following article: http://www.contexo.info/DNA_Basics/polymerase_chain_reaction.htm PCR: the need for a manual and a thermocycler. The PCR technique is currently the gold standard for HIV viral load. Branched DNA / RNA tests are new and not used, and I'm not familiar with them.
FIRST HIV PATIENT NOT DETECTED BY HIV DNA PCR ANTIGEN TEST
